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Single-wavelength two-photon excitation–stimulated emission depletion (SW2PE-STED) superresolution imaging

机译:单波长双光子激发-激发发射损耗(SW2PE-STED)超分辨率成像

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摘要

We developed a new class of two-photon excitation–stimulated emission depletion (2PE-STED) optical microscope. In this work, we show the opportunity to perform superresolved fluorescence imaging, exciting and stimulating the emission of a fluorophore by means of a single wavelength. We show that a widely used red-emitting fluorophore, ATTO647N, can be two-photon excited at a wavelength allowing both 2PE and STED using the very same laser source. This fact opens the possibility to perform 2PE microscopy at four to five times STED-improved resolution, while exploiting the intrinsic advantages of nonlinear excitation.
机译:我们开发了新型的两光子激发-激发发射损耗(2PE-STED)光学显微镜。在这项工作中,我们展示了执行超分辨荧光成像,通过单个波长激发和刺激荧光团发射的机会。我们表明,广泛使用的发红光荧光团ATTO647N可以在允许使用同一激光源的2PE和STED的波长下被双光子激发。这一事实为以2到5倍STED改进的分辨率进行2PE显微术提供了可能性,同时利用了非线性激发的固有优势。

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